Electric yo-yo centrifugation combining with paper-based microfluidic immunoassay chip for inflammatory biomarkers detection in whole blood

Xiaolei Wang; Xiaoli Zheng; Zhihua Song; Dong Lin; Qingling Li; Ji Qi; Jiawen Xiang; Meirong Wang; Lingxin Chen; Bowei Li

doi:10.1016/j.talanta.2022.123883

Electric yo-yo centrifugation combining with paper-based microfluidic immunoassay chip for inflammatory biomarkers detection in whole blood

Talanta. 2023 Feb 1:253:123883. doi: 10.1016/j.talanta.2022.123883. Epub 2022 Sep 6.

Authors

Xiaolei Wang¹, Xiaoli Zheng¹, Zhihua Song², Dong Lin³, Qingling Li¹, Ji Qi³, Jiawen Xiang³, Meirong Wang⁴, Lingxin Chen⁵, Bowei Li⁶

Affiliations

¹ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014, PR China.
² School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, Yantai University, Yantai, 264005, PR China.
³ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China.
⁴ Department of Medical Laboratory, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, 264000, PR China.
⁵ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China. Electronic address: lxchen@yic.ac.cn.
⁶ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China. Electronic address: bwli@yic.ac.cn.

PMID: 36137494
DOI: 10.1016/j.talanta.2022.123883

Abstract

At present, most countries or regions use commercial centrifuges for centrifugation, but this is out of reaching for limited-resource areas. To overcome this problem, a portable electric yo-yo as centrifuge was firstly proposed to obtain serum, and this device can be combined with paper-based analytical devices for enzyme-linked immunosorbent assay (ELISA) analysis from human whole blood. In this study, inflammatory biomarkers C-reactive protein (CRP) and serum amyloid A (SAA) were used as target biomarker to verify the performance of the proposed method. The results shows good performance and their detection limits were determined to be 580 pg/mL for CRP and 800 pg/mL for SAA, respectively. We believe this method provides a new platform of low cost and fast detection for inflammatory biomarkers in the limited-resource settings.

Keywords: Colorimetric; Immunoassay; Inflammation markers; Microfluidic paper-based analytical devices; Point-of-care.