Lactobacillus plantarum ST-III modulates abnormal behavior and gut microbiota in a mouse model of autism spectrum disorder

Min Guo; Ruiying Li; Yuezhu Wang; Shiyu Ma; Yilin Zhang; Sheng Li; Hongfa Zhang; Zhenmin Liu; Chunping You; Huajun Zheng

doi:10.1016/j.physbeh.2022.113965

Lactobacillus plantarum ST-III modulates abnormal behavior and gut microbiota in a mouse model of autism spectrum disorder

Physiol Behav. 2022 Dec 1:257:113965. doi: 10.1016/j.physbeh.2022.113965. Epub 2022 Sep 17.

Authors

Min Guo¹, Ruiying Li², Yuezhu Wang³, Shiyu Ma¹, Yilin Zhang⁴, Sheng Li¹, Hongfa Zhang⁴, Zhenmin Liu⁴, Chunping You⁵, Huajun Zheng⁶

Affiliations

¹ NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, 2140 Xietu Road, Shanghai, China.
² State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
³ Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai and Shanghai Institute for Biomedical and Pharmaceutical Technologies, 250 Bibo Road, Shanghai, China.
⁴ State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.
⁵ State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China. Electronic address: youchunping@brightdairy.com.
⁶ NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, 2140 Xietu Road, Shanghai, China. Electronic address: zhenghj@chgc.sh.cn.

PMID: 36126693
DOI: 10.1016/j.physbeh.2022.113965

Abstract

Probiotic treatment might improve autism spectrum disorder (ASD) behavior. In this study, we investigated the improvement effects of Lactobacillus plantarum ST-III on a mouse model of ASD, which was constructed using triclosan. After two weeks of L. plantarum ST-III oral feeding, autism-like social deficits in male mouse models were ameliorated. L. plantarum ST-III also attenuated the self-grooming and freezing times of female mice. High-throughput sequencing revealed changes in the gut microbiota after L. plantarum ST-III intervention. In the female probiotic group, the abundance of beneficial Lachnospiraceae bacteria increased, whereas that of harmful Alistipes bacteria decreased. Correlation analysis showed that amelioration of abnormal behavior in a mouse model of ASD was related to the involvement of certain metabolic pathways. A reduction in the abundance of Alistipes was involved in stereotyped behavioral improvement. Thus, oral supplementation with L. plantarum ST-III can help improve social behavior in a male mouse model of ASD and contribute to more balanced intestinal homeostasis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autism Spectrum Disorder* / therapy
Disease Models, Animal
Female
Gastrointestinal Microbiome*
Lactobacillus plantarum*
Male
Mice
Probiotics* / therapeutic use