Carbapenemase-producing Enterobacterales (CPEs) strains represent a serious threat to public health. The rapid diffusion of CPEs is of particular concern due to the limited effective treatments available against these multidrug resistant microorganisms. In this study, we characterized the complete genome sequence of Klebsiella pneumoniae strain BO714 coproducing KPC and OXA-181 carbapenemase conferring resistance to all β-lactam/β-lactamase inhibitor combinations (βL-βLICs) and siderophore cephalosporin cefiderocol (CFD). The genome of BO714 has a length of 5,876,068 bp with an average G + C content of 56.96% and a total of 5,878 open reading frames. The KPC-Kp strain BO714 was classified as ST512 and contained a circular chromosome of 5,348,787 bp and three different plasmids, respectively, of 363,560, 112,243, and 51,478 bp. Resistome analysis showed that BO714 harbored different β-lactamase genes including blaCMY-16, blaOXA-10, blaTEM-1, blaSHV-11, blaOXA181, and a novel blaKPC-3 variant named blaKPC-125. KPC-125 differed to KPC-3 by Asp to Ala at position 179 within the Ω-loop region. The genomic characterization of a K. pneumoniae cross-resistant to novel βL-βLICs and CFD improves knowledge regarding the emergence of novel traits of multidrug resistance in CPEs.
Keywords: cefiderocol; cross-resistance; β-lactam/β-lactamase inhibitor combinations.