Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)

Tania Tan; Daniel H D Gray; Charis E Teh

doi:10.1007/978-1-0716-2553-8_8

Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)

Methods Mol Biol. 2022:2543:83-97. doi: 10.1007/978-1-0716-2553-8_8.

Authors

Tania Tan¹, Daniel H D Gray^{1

2}, Charis E Teh^{3

4}

Affiliations

¹ The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
² Department of Medical Biology, The University of Melbourne, Parkville, Australia.
³ The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia. teh.c@wehi.edu.au.
⁴ Department of Medical Biology, The University of Melbourne, Parkville, Australia. teh.c@wehi.edu.au.

PMID: 36087261
DOI: 10.1007/978-1-0716-2553-8_8

Abstract

Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology's use to profile changes in the intrinsic apoptotic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.

Keywords: Apoptosis; BCL-2 family; Cell death; Mass cytometry time-of-flight (CyTOF); Single cell.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Data Analysis*
Flow Cytometry / methods
Signal Transduction*
Staining and Labeling