High-Throughput Binder Confirmation Using Affinity Selection Mass Spectrometry

Methods Mol Biol. 2022:2541:215-223. doi: 10.1007/978-1-0716-2545-3_25.

Abstract

Affinity selection mass spectrometry (AS-MS) was recently applied to a new high-throughput binder confirmation (HTBC) platform. The HTBC-AS-MS platform can assess target engagement for hundreds of chemical series per target and is used at GSK to prioritize synthesis decisions for follow-up organic synthesis of DNA-encoded library technology (ELT) hits.

Keywords: Affinity selection; DNA-encoded library (DEL); ELT; Ligand; Mass spectrometry; Proteins; Screening; Small molecules.

MeSH terms

  • Gene Library
  • Ligands
  • Mass Spectrometry* / methods

Substances

  • Ligands