Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection

Abstract

Delivery of viral vectors to the heart represents a challenging endeavor. Besides vector design, the route of substrate administration is significantly influencing gene delivery success. The selective retrograde venous injection (SRVI) represents one of the most efficient percutaneous delivery strategies for transduction of the anterior left ventricular myocardium. In this chapter, we discuss the advantages and limitations of this vector delivery approach and provide a protocol for selective retrograde venous injection in a preclinical large animal model. As limited transgene expression frequently hampers generation of reliable proof-of-principle data and thus translation, this technique provides a valuable tool to ensure high myocardial transduction in preclinical research.

Keywords: Antegrade occlusion; Cardiovascular disease; Coronary venous delivery; Gene delivery; Gene therapy; Retroinfusion; Selective retrograde venous injection.