In situ generated PtNPs to enhance electrochemiluminescence of multifunctional nanoreactor COP T4VTP6 for AFB1 detection

Xiaoyi Lv; Fei Tan; Tian Miao; Bo Cui; Jianguo Zhang; Yishan Fang; Yizhong Shen

doi:10.1016/j.foodchem.2022.134002

In situ generated PtNPs to enhance electrochemiluminescence of multifunctional nanoreactor COP T₄VTP₆ for AFB₁ detection

Food Chem. 2023 Jan 15:399:134002. doi: 10.1016/j.foodchem.2022.134002. Epub 2022 Aug 25.

Authors

Xiaoyi Lv¹, Fei Tan¹, Tian Miao¹, Bo Cui¹, Jianguo Zhang², Yishan Fang³, Yizhong Shen⁴

Affiliations

¹ School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
² School of Food & Biological Engineering, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China.
³ School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address: fangysh123@qlu.edu.cn.
⁴ School of Food & Biological Engineering, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China. Electronic address: yzshen@hfut.edu.cn.

PMID: 36037690
DOI: 10.1016/j.foodchem.2022.134002

Abstract

Herein, a competitive quenching electrochemiluminescence immunosensor towards aflatoxin B₁ (AFB₁) detection was constructed by in-situ forming platinum nanoparticles (PtNPs) on ECL emitter COP T₄VTP₆ and effective ECL signal quencher Fc-CHO/Phe. In this system, cationic covalent organic polymer COP T₄VTP₆ emitted stronger cathode ECL signal at 765 nm, it acted as an interesting nanoreactor to immobilize PtCl₆^2- through electrostatic adsorption, and directly in situ catalyzed the redox reaction to produce PtNPs without adding any external reducing agent, where PtNPs not only served as the substrate for antibody immobilization, but also played the role of coreaction accelerator to catalyze the production of SO₄^-, significantly improving more stable ECL signal. Moreover, the Fc-CHO/Phe labeled BSA-AFB₁ was used for competitive reaction. Based on the efficient sensing strategy, ECL signal increased accordingly and exhibited linear signal responses with increasing AFB₁, which realized a detection limit of 4.56 fg/mL, providing a promising potential on food analysis.

Keywords: AFB(1); COP T(4)VTP(6); Coreaction accelerator; Electrochemiluminescence; Fc-CHO/Phe; Nanoreactor.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Immunoassay
Limit of Detection
Luminescent Measurements
Metal Nanoparticles*
Nanotechnology
Platinum

Substances

Platinum