The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we present Listeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.
Keywords: Listeria; antimicrobials; biosensors; fluorescence; single-cell analysis.
© 2022 The Authors. Microbiology Open published by John Wiley & Sons Ltd.