Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)

Keiko Iwata; Min-Jue Xie; Paul C Guest; Takaharu Hirai; Hideo Matsuzazki

doi:10.1007/978-1-0716-2395-4_24

Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)

Methods Mol Biol. 2022:2511:321-332. doi: 10.1007/978-1-0716-2395-4_24.

Authors

Keiko Iwata^{1

2

3}, Min-Jue Xie^{4

5

6}, Paul C Guest⁷, Takaharu Hirai⁸, Hideo Matsuzazki^{4

5

6}

Affiliations

¹ Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan. kiwata@u-fukui.ac.jp.
² United Graduate School of Child Development, Osaka University, Osaka, Japan. kiwata@u-fukui.ac.jp.
³ Life Science Innovation Center, University of Fukui, Fukui, Japan. kiwata@u-fukui.ac.jp.
⁴ Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan.
⁵ United Graduate School of Child Development, Osaka University, Osaka, Japan.
⁶ Life Science Innovation Center, University of Fukui, Fukui, Japan.
⁷ Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
⁸ Department of Psychiatric and Mental Health Nursing, School of Nursing, University of Fukui, Fukui, Japan.

PMID: 35838971
DOI: 10.1007/978-1-0716-2395-4_24

Abstract

Inflammatory diseases caused by infectious agents such as the SARS-CoV-2 virus can lead to impaired reductive-oxidative (REDOX) balance and disrupted mitochondrial function. Peripheral blood mononuclear cells (PBMCs) provide a useful model for studying the effects of inflammatory diseases on mitochondrial function but can be limited by the need to store these cells by cryopreservation prior to assay. Here, we describe a method for improving and determining PBMC viability with normalization of values to number of living cells. The approach can be applied not only to PBMC samples derived from patients with diseases marked by an altered inflammatory response such as viral infections.

Keywords: COVID-19; Cryopreservation; Inflammatory disease; Mitochondrial respiration; PBMCs; SARS-CoV-2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19*
Cryopreservation / methods
Humans
Leukocytes, Mononuclear* / metabolism
Mitochondria
Respiration
SARS-CoV-2