The complete chloroplast genome of Pulsatilla campanella Fischer ex Krylov. (Ranunculaceae, Pulsatilla Miller)

Hefei Xue; Yueyue Song; Yanyun Yang; Che Bian; Liang Xu; Tingguo Kang

doi:10.1080/23802359.2022.2087556

The complete chloroplast genome of Pulsatilla campanella Fischer ex Krylov. (Ranunculaceae, Pulsatilla Miller)

Mitochondrial DNA B Resour. 2022 Jun 23;7(6):1126-1128. doi: 10.1080/23802359.2022.2087556. eCollection 2022.

Authors

Hefei Xue^{1

2}, Yueyue Song¹, Yanyun Yang¹, Che Bian¹, Liang Xu¹, Tingguo Kang¹

Affiliations

¹ School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
² Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde, China.

Abstract

The complete chloroplast genome of Pulsatilla campanella Fischer ex Krylov was sequenced and reported for the first time. The length of the entire circular genome was 162,322 bp, and the GC content was 37.4%. There were 133 genes annotated, including 89 known protein-coding genes, 36 tRNAs, and 8 rRNAs. The complete chloroplast genome of P.campanella has consisted of two inverted repeat regions (IRs), a large single-copy region (LSC 82,087 bp), and a small single-copy region (SSC 17,497 bp). The phylogenetic tree was built based on 29 species, using the maximum-likelihood method. The results showed that P.campanella was clustered on the same branch with a variety of Pulsatilla plants. The data reveal the genetic relationship between the selected species and provide information for subsequent plant classification.

Keywords: Pulsatilla campanella; Ranunculaceae; chloroplast genome; phylogenetic.

Grants and funding

This research was funded by Liaoning BaiQianWan Talents Program; Natural Science Foundation of Hebei Province [H2019406067]; 2019 Liaoning Provincial Department of Education Scientific Research Project [L201942]; National Key Research and Development in the 13th Five-Year Plan [2018YFC1708200]; Major Special Fund for Science and Technology of Inner Mongolia Autonomous Region [2019ZD004]; Natural Science Fund Project of Liaoning Province [2020-MS-224].