Repurposing a DNA-Repair Enzyme for Targeted Protein Degradation

Callie Fredlender; Samantha Levine; Jan Zimak; Robert C Spitale

doi:10.1002/cbic.202200053

Repurposing a DNA-Repair Enzyme for Targeted Protein Degradation

Chembiochem. 2022 Oct 6;23(19):e202200053. doi: 10.1002/cbic.202200053. Epub 2022 Jun 24.

Authors

Callie Fredlender¹, Samantha Levine¹, Jan Zimak¹, Robert C Spitale^{1

2}

Affiliations

¹ Department of Pharmaceutical Sciences, University of California, Irvine, 2403 Natural Sciences I, 92617, Irvine, CA, USA.
² Department of Chemistry, University of California, Irvine, 2403 Natural Sciences I, 92617, Irvine., CA, USA.

Abstract

Herein we present the exploration of the utility of DNA demethylase enzymes for targeted protein degradation. Novel benzylguanine substrates are characterized for their ability to control protein degradation in cells. Our data demonstrate the utility of this approach to degrade fusion proteins in different localizations within living cells.

Keywords: SNAP tag; adamantyl; protein degron.

MeSH terms

DNA Repair Enzymes*
Proteolysis
Recombinant Fusion Proteins

Substances

Recombinant Fusion Proteins
DNA Repair Enzymes

Grants and funding

P30 CA062203/CA/NCI NIH HHS/United States