Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent models

Mariana De Niz; Luisa M Figueiredo

doi:10.1016/j.xpro.2022.101450

Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent models

STAR Protoc. 2022 Jun 13;3(2):101450. doi: 10.1016/j.xpro.2022.101450. eCollection 2022 Jun 17.

Authors

Mariana De Niz¹, Luisa M Figueiredo¹

Affiliation

¹ Instituto de Medicina Molecular - João lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal.

Abstract

Intravital microscopy (IVM) involves surgical procedures to expose the internal organs of live anesthetized animals to visualize fluorescently labeled components in situ, in vivo at subcellular resolution. Here, we provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei-host interactions in ten mammalian organs and in systemic circulation. We describe intraperitoneal or intradermal injection of mice with T.brucei. We then detail surgical procedures to prepare ten organs for IVM, followed by imaging of host-T. brucei interactions. For complete details on the use and execution of this protocol, please refer to De Niz et al. (2021).

Keywords: Cell Biology; Microbiology; Microscopy; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Intravital Microscopy / methods
Mice
Rodentia
Trypanosoma brucei brucei*