RNA:DNA hybrids are generated naturally behind the elongating RNA polymerase as a transcriptional intermediate. However, prolonged persistence of these structures challenges the integrity of the genome by creating R-loops and by interfering with DNA replication and other chromatin related processes. Precise mapping and characterization of their distribution across the genome has been a major challenge to understand the genesis of RNA:DNA hybrids and their conversion into genotoxic intermediates. Here we provide the detailed protocol for mapping RNA:DNA hybrid across the Saccharomyces cerevisiae genome.
Keywords: DRIP-chip; R-loop; RNA:DNA hybrid; S9.6 antibody.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.