Control of Root Stem Cell Differentiation and Lateral Root Emergence by CLE16/17 Peptides in Arabidopsis

Lihua Zhang; Yi Yang; Changqing Mu; Mingyu Liu; Takashi Ishida; Shinichiro Sawa; Yuxian Zhu; Limin Pi

doi:10.3389/fpls.2022.869888

Control of Root Stem Cell Differentiation and Lateral Root Emergence by CLE16/17 Peptides in Arabidopsis

Front Plant Sci. 2022 Apr 18:13:869888. doi: 10.3389/fpls.2022.869888. eCollection 2022.

Authors

Lihua Zhang¹, Yi Yang², Changqing Mu², Mingyu Liu², Takashi Ishida^{3

4}, Shinichiro Sawa³, Yuxian Zhu^{1

2}, Limin Pi²

Affiliations

¹ State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
² State Key Laboratory of Hybrid Rice, Institute for Advanced Studies, Wuhan University, Wuhan, China.
³ Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
⁴ International Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, Kumamoto, Japan.

Abstract

Secreted peptide-mediated cell-to-cell communication plays a crucial role in the development of multicellular organisms. A large number of secreted peptides have been predicated by bioinformatic approaches in plants. However, only a few of them have been functionally characterized. In this study, we show that two CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides CLE16/17 are required for both stem cell differentiation and lateral root (LR) emergence in Arabidopsis. We further demonstrate that the CLE16/17 peptides act through the CLAVATA1-ARABIDOPSIS CRINKLY4 (CLV1-ACR4) protein kinase complex in columella stem cell (CSC) differentiation, but not in LR emergence. Furthermore, we show that CLE16/17 promote LR emergence probably via activating the expression of HAESA/HAESA-LIKE2 (HAE/HSL2) required for cell wall remodeling. Collectively, our results reveal a CLV1-ACR4-dependent and -independent dual-function of the CLE16/17 peptides in root development.

Keywords: Arabidopsis; CLE16/17; columella stem cell; lateral root emergence; stem cell differentiation.