Leukocyte telomere dynamics across gestation in uncomplicated pregnancies and associations with stress

Danielle M Panelli; Stephanie A Leonard; Ronald J Wong; Martin Becker; Jonathan A Mayo; Erica Wu; Anna I Girsen; Ian H Gotlib; Nima Aghaeepour; Maurice L Druzin; Gary M Shaw; David K Stevenson; Katherine Bianco

doi:10.1186/s12884-022-04693-0

Leukocyte telomere dynamics across gestation in uncomplicated pregnancies and associations with stress

BMC Pregnancy Childbirth. 2022 May 2;22(1):381. doi: 10.1186/s12884-022-04693-0.

Authors

Affiliations

¹ Department of Obstetrics and Gynecology, Stanford University, 453 Quarry Road, Palo Alto, CA, 94304, USA. dpanelli@stanford.edu.
² Department of Obstetrics and Gynecology, Stanford University, 453 Quarry Road, Palo Alto, CA, 94304, USA.
³ Department of Pediatrics, Stanford University, Stanford, CA, USA.
⁴ Department of Anesthesiology, Perioperative, and Pain Medicine, Stanford University, Stanford, CA, USA.
⁵ Department of Biomedical Data Science, Stanford University, Stanford, CA, USA.
⁶ Department of Psychology, Stanford University, Stanford, CA, USA.

Abstract

Background: Short leukocyte telomere length is a biomarker associated with stress and morbidity in non-pregnant adults. Little is known, however, about maternal telomere dynamics in pregnancy. To address this, we examined changes in maternal leukocyte telomere length (LTL) during uncomplicated pregnancies and explored correlations with perceived stress.

Methods: In this pilot study, maternal LTL was measured in blood collected from nulliparas who delivered live, term, singleton infants between 2012 and 2018 at a single institution. Participants were excluded if they had diabetes or hypertensive disease. Samples were collected over the course of pregnancy and divided into three time periods: < 20^0/7 weeks (Timepoint 1); 20^1/7 to 36^6/7 weeks (Timepoint 2); and 37^0/7 to 9-weeks postpartum (Timepoint 3). All participants also completed a survey assessing a multivariate profile of perceived stress at the time of enrollment in the first trimester. LTL was measured using quantitative polymerase chain reaction (PCR). Wilcoxon signed-rank tests were used to compare LTL differences within participants across all timepoint intervals. To determine whether mode of delivery affected LTL, we compared postpartum Timepoint 3 LTLs between participants who had vaginal versus cesarean birth. Secondarily, we evaluated the association of the assessed multivariate stress profile and LTL using machine learning analysis.

Results: A total of 115 samples from 46 patients were analyzed. LTL (mean ± SD), expressed as telomere to single copy gene (T/S) ratios, were: 1.15 ± 0.26, 1.13 ± 0.23, and 1.07 ± 0.21 for Timepoints 1, 2, and 3, respectively. There were no significant differences in LTL between Timepoints 1 and 2 (LTL T/S change - 0.03 ± 0.26, p = 0.39); 2 and 3 (- 0.07 ± 0.29, p = 0.38) or Timepoints 1 and 3 (- 0.07 ± 0.21, p = 0.06). Participants who underwent cesareans had significantly shorter postpartum LTLs than those who delivered vaginally (T/S ratio: 0.94 ± 0.12 cesarean versus 1.12 ± 0.21 vaginal, p = 0.01). In secondary analysis, poor sleep quality was the main stress construct associated with shorter Timepoint 1 LTLs (p = 0.02) and shorter mean LTLs (p = 0.03).

Conclusions: In this cohort of healthy pregnancies, maternal LTLs did not significantly change across gestation and postpartum LTLs were shorter after cesarean than after vaginal birth. Significant associations between sleep quality and short LTLs warrant further investigation.

Keywords: Leukocyte telomere length; Maternal mental health; Pregnancy; Psychosocial stress; Sleep.

MeSH terms

Adult
Cohort Studies
Female
Humans
Leukocytes
Pilot Projects
Pregnancy
Telomere Shortening*
Telomere*

Grants and funding

R35 GM138353/GM/NIGMS NIH HHS/United States