Transcription factor 7-like 2 single nucleotide polymorphisms rs290487 and rs290481 are associated with dyslipidemia in the Balinese population

Prisca C Limardi; Sukma Oktavianthi; Lidwina Priliani; Retno Lestari; Made Ratna Saraswati; Ketut Suastika; Safarina G Malik

doi:10.7717/peerj.13149

Transcription factor 7-like 2 single nucleotide polymorphisms rs290487 and rs290481 are associated with dyslipidemia in the Balinese population

PeerJ. 2022 Mar 22:10:e13149. doi: 10.7717/peerj.13149. eCollection 2022.

Authors

Prisca C Limardi^{1

2}, Sukma Oktavianthi¹, Lidwina Priliani¹, Retno Lestari², Made Ratna Saraswati³, Ketut Suastika³, Safarina G Malik¹

Affiliations

¹ Genome Diversity and Diseases Laboratory, Eijkman Institute for Molecular Biology, National Research and Innovation Agency, Jakarta, DKI Jakarta, Indonesia.
² Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, West Java, Indonesia.
³ Division of Endocrinology and Metabolism, Department of Internal Medicine, Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia.

Abstract

Background: Dyslipidemia is one of the major risks for the development of cardiovascular diseases which has been the leading cause of death in developing countries. Previously, common polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene have been associated with altered lipid profiles. In this study, we investigated the associations of TCF7L2 SNPs, rs290487 and rs290481, with dyslipidemia and altered lipid profile in the Balinese.

Methods: A total of 565 subjects from four locations in the Bali Province, Indonesia, were recruited. Serum lipid concentrations (triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC)) were measured using standard protocol. SNP genotyping was done using the amplification refractory system mutation polymerase chain reaction (ARMS-PCR) method.

Results: We found the shifted major/minor allele frequencies of both SNPs (0.56 for rs290487 T allele, 0.53 for rs290481 T allele) in the Balinese, as compared to dbSNP. The rs290487 and rs290481 C alleles were significantly associated with dyslipidemia, particularly high TC and high LDL-C. These associations were independent of age, sex, population, obesity, diabetes mellitus, and high TyG index as a proxy for insulin resistance. The haplotype CC also showed similar association with these traits. Our findings indicate that TCF7L2 polymorphisms are associated with dyslipidemia and altered lipid profile in the Balinese.

Keywords: Dyslipidemia; Lipid profiles; SNPs; TCF7L2; rs290481; rs290487.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cholesterol, LDL
Dyslipidemias* / genetics
Genetic Predisposition to Disease / genetics
Humans
Polymorphism, Single Nucleotide* / genetics
T Cell Transcription Factor 1

Substances

T Cell Transcription Factor 1
Cholesterol, LDL

Grants and funding

This research was supported by a block grant from the Government of Republic of Indonesia through the Ministry of Research and Technology for the Eijkman Institute for Molecular Biology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.