The therapeutic capacity of bone marrow MSC-derived extracellular vesicles in Achilles tendon healing is passage-dependent and indicated by specific glycans

Yuta Hayashi; Dilimulati Yimiti; Yohei Sanada; Chenyang Ding; Takenori Omoto; Toshihiko Ogura; Tomoyuki Nakasa; Masakazu Ishikawa; Keiko Hiemori; Hiroaki Tateno; Shigeru Miyaki; Nobuo Adachi

doi:10.1002/1873-3468.14333

The therapeutic capacity of bone marrow MSC-derived extracellular vesicles in Achilles tendon healing is passage-dependent and indicated by specific glycans

FEBS Lett. 2022 Apr;596(8):1047-1058. doi: 10.1002/1873-3468.14333. Epub 2022 Mar 29.

Authors

Yuta Hayashi^{1

2}, Dilimulati Yimiti¹, Yohei Sanada^{1

3}, Chenyang Ding¹, Takenori Omoto¹, Toshihiko Ogura⁴, Tomoyuki Nakasa^{1

3}, Masakazu Ishikawa^{1

5}, Keiko Hiemori⁶, Hiroaki Tateno⁶, Shigeru Miyaki^{1

3}, Nobuo Adachi¹

Affiliations

¹ Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Japan.
² Department of Musculoskeletal Traumatology and Reconstructive surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Japan.
³ Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Japan.
⁴ Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
⁵ Department of Artificial Joints and Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Japan.
⁶ Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.

PMID: 35294042
DOI: 10.1002/1873-3468.14333

Abstract

The therapeutic potential of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) for various diseases and tissue repair is attracting attention. Here, EVs from conditioned medium of human bone marrow MSCs at passage 5 (P5) and passage 12 (P12) were analysed using mouse Achilles tendon rupture model and lectin microarray. P5 MSC-EVs accelerated Achilles tendon healing compared with P12 MSC-EVs. Fucose-specific lectin TJA-II was indicated as a glycan marker for therapeutic MSC-EVs. The present study demonstrated that early passaged MSC-EVs promote Achilles tendon healing compared with senescent MSC-EVs. Glycans on MSC-EVs might provide useful tools to establish a quality control and isolation system for therapeutic MSC-EVs in regenerative medicine.

Keywords: achilles tendon; extracellular vesicles; glycome; lectin array; mesenchymal stem cell; regenerative medicine; tendon healing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Achilles Tendon*
Animals
Bone Marrow
Disease Models, Animal
Extracellular Vesicles*
Mesenchymal Stem Cells*
Mice
Polysaccharides

Substances

Polysaccharides