Saliva cortisol diurnal variation and stress responses in term and preterm infants

Arch Dis Child Fetal Neonatal Ed. 2022 Sep;107(5):558-564. doi: 10.1136/archdischild-2021-321593. Epub 2022 Mar 7.

Abstract

Objective: To determine if preterm birth is associated with adaptation of the hypothalamic-pituitary-adrenal (HPA) axis and whether HPA axis programming relates to the degree of prematurity (defined as extremely preterm birth at <28 weeks or very preterm birth at 28-32 weeks gestation).

Design: This study reports findings from a prospective birth cohort. Saliva cortisol concentrations were measured prevaccination and postvaccination, and in the morning and evening, at 4 months chronological age.

Setting: Infants born at a single Scottish hospital.

Participants: 45 term-born, 42 very preterm and 16 extremely preterm infants.

Outcomes: Cortisol stress response to vaccination (postvaccination minus prevaccination cortisol concentrations), diurnal slope (log-transformed morning minus log-transformed evening cortisol values) and mean log-transformed daily cortisol.

Results: Compared with infants born at term, infants born extremely preterm had a blunted cortisol response to vaccination (5.8 nmol/L vs 13.1 nmol/L, difference in means: -7.3 nmol/L, 95% CI -14.0 to -0.6) and a flattened diurnal slope (difference in geometric means: -72.9%, 95% CI -87.1 to -42.8). In contrast, the cortisol response to vaccination (difference in means -2.7 nmol/L, 95% CI -7.4 to 2.0) and diurnal slope at 4 months (difference in geometric means: -33.6%, 95% CI -62.0 to 16.0) did not differ significantly in infants born very preterm compared with infants born at term.

Conclusions: Infants born extremely preterm have blunted cortisol reactivity and a flattened diurnal slope. These patterns of HPA axis regulation are commonly seen after childhood adversity and could contribute to later metabolic and neurodevelopmental phenotypes observed in this population.

Keywords: endocrinology; infant development; neonatology.

MeSH terms

  • Female
  • Humans
  • Hydrocortisone*
  • Hypothalamo-Hypophyseal System / metabolism
  • Infant, Extremely Premature
  • Infant, Newborn
  • Pituitary-Adrenal System / metabolism
  • Premature Birth*
  • Prospective Studies
  • Saliva

Substances

  • Hydrocortisone