Effect of an inulin-type fructan from Platycodon grandiflorum on the intestinal microbiota in rats exposed to PM2.5

Shan Shan; Yi Xiong; Jianguo Guo; Mengyao Liu; Xin Gao; Xinjing Fu; Deyong Zeng; Chen Song; Yingchun Zhang; Dayodu Cheng; Weihong Lu

doi:10.1016/j.carbpol.2022.119147

Effect of an inulin-type fructan from Platycodon grandiflorum on the intestinal microbiota in rats exposed to PM2.5

Carbohydr Polym. 2022 May 1:283:119147. doi: 10.1016/j.carbpol.2022.119147. Epub 2022 Jan 17.

Authors

Shan Shan¹, Yi Xiong¹, Jianguo Guo², Mengyao Liu¹, Xin Gao¹, Xinjing Fu², Deyong Zeng¹, Chen Song¹, Yingchun Zhang³, Dayodu Cheng³, Weihong Lu⁴

Affiliations

¹ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin, China.
² Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing 100021, China.
³ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
⁴ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin, China; Institute of Extreme Environment Nutrition and Protection, Harbin Institute of Technology, Harbin, China. Electronic address: lwh@hit.edu.cn.

PMID: 35153025
DOI: 10.1016/j.carbpol.2022.119147

Abstract

In this study, an inulin-type fructan (PGPI-1-a) was isolated from the roots of Platycodon grandiflorum. PGPI-1-a was composed of (2 → 1)-linked β-D-fructofuranose (Fruf) and a terminal α-d-glucopyranose (Glcp) with a molecular weight of 12.1 kDa. PM2.5 exposure has brought a great threat to human health in recent years. Therefore, this study explored the effect of PGPI-1-a on the intestinal microbial community structure of rats exposed to PM2.5 using the animal model of PM2.5 inhalation exposure. The results showed that PGPI-1-a could regulate the intestinal microbiota by partly restoring the perturbed levels of Peptoniphilaceae_[G-2] and Lachnospiraceae_[G-2] caused by PM2.5 exposure. In addition, the relative abundance of Butyrivibrio, a butyric acid-producing genera, significantly increased after PGPI-1-a intervention. These results indicated that PGPI-1-a could improve the imbalance of intestinal microbiota due to PM2.5 exposure to a certain extent.

Keywords: Intestinal microbiota; Inulin-type fructan; PM2.5; Platycodon grandiflorum.

MeSH terms

Animals
Butyrivibrio / drug effects
Clostridiales / drug effects
Fructans / analysis
Fructans / pharmacology*
Gastrointestinal Microbiome / drug effects*
Humans
Inulin / analysis
Inulin / pharmacology*
Male
Microbiota
Molecular Weight
Particulate Matter / adverse effects*
Plant Roots / chemistry
Platycodon / chemistry*
Rats

Substances

Fructans
Particulate Matter
Inulin