NK cell frequencies, function and correlates to vaccine outcome in BNT162b2 mRNA anti-SARS-CoV-2 vaccinated healthy and immunocompromised individuals

Mol Med. 2022 Feb 8;28(1):20. doi: 10.1186/s10020-022-00443-2.

Abstract

Adaptive immune responses have been studied extensively in the course of mRNA vaccination against COVID-19. Considerably fewer studies have assessed the effects on innate immune cells. Here, we characterized NK cells in healthy individuals and immunocompromised patients in the course of an anti-SARS-CoV-2 BNT162b2 mRNA prospective, open-label clinical vaccine trial. See trial registration description in notes. Results revealed preserved NK cell numbers, frequencies, subsets, phenotypes, and function as assessed through consecutive peripheral blood samplings at 0, 10, 21, and 35 days following vaccination. A positive correlation was observed between the frequency of NKG2C+ NK cells at baseline (Day 0) and anti-SARS-CoV-2 Ab titers following BNT162b2 mRNA vaccination at Day 35. The present results provide basic insights in regards to NK cells in the context of mRNA vaccination, and have relevance for future mRNA-based vaccinations against COVID-19, other viral infections, and cancer.Trial registration: The current study is based on clinical material from the COVAXID open-label, non-randomized prospective clinical trial registered at EudraCT and clinicaltrials.gov (no. 2021-000175-37). Description: https://clinicaltrials.gov/ct2/show/NCT04780659?term=2021-000175-37&draw=2&rank=1 .

Keywords: Anti-SARS-CoV-2 antibodies; BNT162b2 mRNA vaccine; COVID-19; Clinical trial; Innate immunity; NK cells; NKG2C; SARS-CoV-2; mRNA vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Viral / immunology
  • BNT162 Vaccine / administration & dosage
  • BNT162 Vaccine / immunology*
  • COVID-19 / epidemiology
  • COVID-19 / immunology*
  • COVID-19 / virology
  • COVID-19 Vaccines / administration & dosage
  • COVID-19 Vaccines / immunology*
  • Female
  • Flow Cytometry
  • Humans
  • Immunocompromised Host / immunology*
  • Killer Cells, Natural / immunology*
  • Killer Cells, Natural / metabolism
  • Lymphocyte Count
  • Male
  • Middle Aged
  • NK Cell Lectin-Like Receptor Subfamily C / immunology
  • NK Cell Lectin-Like Receptor Subfamily C / metabolism
  • Outcome Assessment, Health Care / methods
  • Outcome Assessment, Health Care / statistics & numerical data
  • Pandemics / prevention & control
  • SARS-CoV-2 / immunology*
  • SARS-CoV-2 / physiology
  • Vaccination / methods
  • Vaccination / statistics & numerical data
  • Young Adult

Substances

  • Antibodies, Viral
  • COVID-19 Vaccines
  • KLRC2 protein, human
  • NK Cell Lectin-Like Receptor Subfamily C
  • BNT162 Vaccine

Associated data

  • ClinicalTrials.gov/NCT04780659