Characterization of transcriptional landscape in bone marrow-derived mesenchymal stromal cells treated with aspirin by RNA-seq

Xinpeng Liu; Yuanbo Zhan; Wenxia Xu; Lixue Liu; Xiaoyao Liu; Junlong Da; Kai Zhang; Xinjian Zhang; Jianqun Wang; Ziqi Liu; Han Jin; Bin Zhang; Ying Li

doi:10.7717/peerj.12819

Characterization of transcriptional landscape in bone marrow-derived mesenchymal stromal cells treated with aspirin by RNA-seq

PeerJ. 2022 Jan 24:10:e12819. doi: 10.7717/peerj.12819. eCollection 2022.

Authors

Xinpeng Liu¹, Yuanbo Zhan^{1

2}, Wenxia Xu¹, Lixue Liu¹, Xiaoyao Liu¹, Junlong Da¹, Kai Zhang¹, Xinjian Zhang¹, Jianqun Wang¹, Ziqi Liu¹, Han Jin¹, Bin Zhang^{1

3}, Ying Li¹

Affiliations

¹ Heilongjiang Provincial Key Laboratory of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
² The Second Affiliated Hospital of Harbin Medical University, Department of Periodontology and Oral Mucosa, Harbin, China.
³ Heilongjiang Academy of Medical Sciences, Harbin, China.

Abstract

Introduction: Aspirin is a common antipyretic, analgesic, and anti-inflammatory drug, which has been reported to extend life in animal models and application in the treatment of aging-related diseases. However, it remains unclear about the effects of aspirin on bone marrow-derived mesenchymal stromal cells (BM-MSCs). Here, we aimed to analyze the influence of aspirin on senescence and young BM-MSCs.

Methods: BM-MSCs were serially passaged to construct a replicative senescence model. SA-β-gal staining, PCR, western blot, and RNA-sequencing were performed on BM-MSCs with or without aspirin treatment, to examine aspirin's impact on bone marrow-derived mesenchymal stem cells.

Results: SA-β-gal staining, PCR, and western blot revealed that aspirin could alleviate the cellular expression of senescence-related indicators of BM-MSCs, including a decrease of SA-β-gal-positive cells and staining intensity, and downregulation of p16, p21, and p53 expression after aspirin treatment. RNA-sequencing results shown in the biological processes related to aging, aspirin could influence cellular immune response and lipid metabolism.

Conclusion: The efficacy of aspirin for retarding senescence of BM-MSCs was demonstrated. Our study indicated that the mechanisms of this delay might involve influencing immune response and lipid metabolism.

Keywords: Aspirin; BM-MSCs; Cell senescence; Immune response; Lipid metabolism; RNA-seq.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aspirin / pharmacology
Bone Marrow
Cells, Cultured
Cellular Senescence* / genetics
Mesenchymal Stem Cells*
RNA / metabolism
RNA-Seq

Substances

Aspirin
RNA

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 81801040, 82001488, 81870736), the Natural Science Foundation of Heilongjiang Province of China (Grant Nos. LH2020H057), and the Harbin Medical University Innovation Fund Clinical Project (Grant Nos. 2019-KYYWF-0347). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.