Clinical Evaluation of a Novel Insulin Immunosensor

J Diabetes Sci Technol. 2023 Jul;17(4):1038-1048. doi: 10.1177/19322968221074406. Epub 2022 Feb 4.

Abstract

Background: The estimation of available active insulin remains a limitation of automated insulin delivery systems. Currently, insulin pumps calculate active insulin using mathematical decay curves, while quantitative measurements of insulin would explicitly provide person-specific PK insulin dynamics to assess remaining active insulin more accurately, permitting more effective glucose control.

Methods: We performed the first clinical evaluation of an insulin immunosensor chip, providing near real-time measurements of insulin levels. In this study, we sought to determine the accuracy of the novel insulin sensor and assess its therapeutic risk and benefit by presenting a new tool developed to indicate the potential therapeutic consequences arising from inaccurate insulin measurements.

Results: Nine adult participants with type-1 diabetes completed the study. The change from baseline in immunosensor-measured insulin levels was compared with values obtained by standard enzyme-linked immunosorbant assay (ELISA) after preprandial injection of insulin. The point-of-care quantification of insulin levels revealed similar temporal trends as those from the laboratory insulin ELISA. The results showed that 70% of the paired immunosensor-reference values were concordant, which suggests that the patient could take action safely based on insulin concentration obtained by the novel sensor.

Conclusions: This proposed technology and preliminary feasibility evaluation show encouraging results for near real-time evaluation of insulin levels, with the potential to improve diabetes management. Real-time measurements of insulin provide person-specific insulin dynamics that could be used to make more informed decisions regarding insulin dosing, thus helping to prevent hypoglycemia and improve diabetes outcomes.

Keywords: immunosensor; insulin measurement; point-of-care; type-1 diabetes.

MeSH terms

  • Adult
  • Biosensing Techniques*
  • Blood Glucose / analysis
  • Blood Glucose Self-Monitoring / methods
  • Diabetes Mellitus, Type 1* / drug therapy
  • Humans
  • Immunoassay
  • Insulin
  • Insulin, Regular, Human / therapeutic use

Substances

  • Insulin
  • Blood Glucose
  • Insulin, Regular, Human