Circular RNA circ_0005835 promotes promoted neural stem cells proliferation and differentiate to neuron and inhibits inflammatory cytokines levels through miR-576-3p in Alzheimer's disease

Abstract

Alzheimer's disease (AD) is the most frequent neurodegenerative disease and it is difficult to have an effective and simple method for AD early diagnosis. CircRNAs (circular RNAs) are novel discovered non-coding endogenous RNAs that affect cell apoptosis, differentiation, growth, metabolism, and metastasis. Recently, it has reported that circ_0005835 was one upregulated circRNA in the AD patients. However, the function role of circ_0005835 remains unknown. In our study, we found that circ_0005835 was upregulated in AD patients and cell models. Knockdown of circ_0005835 could downregulate neuroinflammation in BV2 cells. Moreover, knockdown of circ_0005835 promoted neural stem cells (NSC) proliferation and differentiate to neuron. These data mean that circ_0005835 plays important role in the development of AD. The miR-576-3p expression in serum was downregulated in the AD group compared to the health control group. Consistently, the level of circ_0005835 was overexpressed in the Aβ-treated in both SH-SY5Y and BV2 cells. Moreover, the expression of miR-576-3p was negatively correlated with circ_0005835 in AD patients. In addition, we performed the rescued experiments to show that knockdown of circ_0005835 could downregulate neuroinflammation through sponging miR-576-3p in BV2 cells. Inhibition of circ_0005835 promoted NSC proliferation and differentiate to neuron via sponging miR-576-3p. These data suggested that circ_0005835 promoted AD development through regulating miR-576-3p expression.

Keywords: AD; Alzheimer’s disease; circ_0005835; miR-576-3p.