Ascorbic Acid-Loaded Apoferritin-Assisted Carbon Dot-MnO2 Nanocomposites for the Selective and Sensitive Detection of Trypsin

Qingqing Tan; Ruirui Zhang; Weisu Kong; Fengli Qu; Limin Lu

doi:10.1021/acsabm.8b00235

Ascorbic Acid-Loaded Apoferritin-Assisted Carbon Dot-MnO₂ Nanocomposites for the Selective and Sensitive Detection of Trypsin

ACS Appl Bio Mater. 2018 Sep 17;1(3):777-782. doi: 10.1021/acsabm.8b00235. Epub 2018 Sep 4.

Authors

Qingqing Tan¹, Ruirui Zhang², Weisu Kong¹, Fengli Qu¹, Limin Lu³

Affiliations

¹ College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, People's Republic of China.
² Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
³ Institute of Functional Materials and Agricultural Applied Chemistry, College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.

PMID: 34996168
DOI: 10.1021/acsabm.8b00235

Abstract

A novel "turn-on" fluorescent trypsin detection platform dependant on carbon dot-MnO₂ (CD-MnO₂) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO₂ nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.

Keywords: MnO2 nanosheets; apoferritin; carbon dots; fluorescence; fluorescence resonance energy transfer; trypsin.