Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence

Methods Mol Biol. 2022:2435:107-127. doi: 10.1007/978-1-0716-2014-4_8.

Abstract

We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies.

Keywords: Immune markers; Multiplex IF; Opal™; Quantification algorithms; Vectra®.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fluorescent Antibody Technique
  • Mice
  • Paraffin Embedding
  • Staining and Labeling
  • Tumor Microenvironment*