Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis

Drug Des Devel Ther. 2021 Dec 21:15:5075-5086. doi: 10.2147/DDDT.S340410. eCollection 2021.

Abstract

Objective: To investigate the correlation of the Val109Asp polymorphism of the omentin-1 gene with the risk and severity of knee osteoarthritis (KOA) in a Chinese Han population.

Methods: This study enrolled 383 patients with primary KOA and 460 healthy controls. The genotypes were determined by the detection of single nucleotide polymorphism. To explore the interaction between omentin-1 gene polymorphism and obesity and age, the body mass index (BMI) of 25 kg/m2 and the age of 55 years old were preset as the cut-off value of stratified analysis. Furthermore, enzyme-linked immunosorbent assay was used to determine the levels of omentin-1, interleukin (IL)-1β, IL-6 in peripheral blood and synovial fluid and the contents of IL-1β, IL-6, metalloproteinase (MMP)-13 and collagen (COL)-II in the supernatant of knee joint cartilage tissue.

Results: The Val109Asp polymorphism of the omentin-1 gene showed no obvious correlation with KOA. Compared with Asp/Asp genotype carriers with BMI <25 kg/m2 and age <55 years old, Val109 allele carriers with BMI≥25 kg/m2 and age ≥55 years old had obviously increased risk of KOA (adjusted OR = 1.416, p = 0.042; adjusted OR = 1.735, p = 0.038, respectively). In the KOA group, only the omentin-1 levels were significantly lower in the plasma and synovial fluid of Ala/Ala genotype carriers than in those of Asp/Asp genotype carriers. Meanwhile, the proportion of patients with moderate-severe K-L Classification, the levels of IL-1β, IL-6 in synovial fluid and the expression levels of IL-1β, IL-6 and MMP-13 in cartilage tissue significantly increased (p < 0.05). By contrast, the expression level of COL-II in cartilage tissue significantly decreased (p < 0.05).

Conclusions: The Val109Asp polymorphism of the omentin-1 gene may not be the primary pathogenic factor of KOA in Chinese. The Val/Val genotype can be regarded as a potential biomarker for the risk of KOA progression.

Keywords: Chinese; biomarker; knee osteoarthritis; obesity; omentin-1; single nucleotide polymorphism.

MeSH terms

  • Aged
  • Biomarkers / metabolism
  • Body Mass Index
  • Case-Control Studies
  • China / epidemiology
  • Cytokines / genetics*
  • Cytokines / metabolism
  • Female
  • GPI-Linked Proteins / genetics
  • GPI-Linked Proteins / metabolism
  • Genotype
  • Humans
  • Incidence
  • Lectins / genetics*
  • Lectins / metabolism
  • Male
  • Middle Aged
  • Osteoarthritis, Knee / epidemiology
  • Osteoarthritis, Knee / genetics*
  • Polymorphism, Single Nucleotide
  • Valine / genetics*

Substances

  • Biomarkers
  • Cytokines
  • GPI-Linked Proteins
  • ITLN1 protein, human
  • Lectins
  • Valine

Grants and funding

This study was supported by the Key Research and Development Projects of Anhui Province (Grant No. 1804h08020228) and the Natural Science Foundation of Anhui Province in China (2108085MH269). The funding body had no role in the design of the study, or the collection, analysis, and interpretation of data, or in writing the manuscript.