Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

Keiko Sakamoto; Shubham Goel; Atsuko Funakoshi; Tetsuya Honda; Keisuke Nagao

doi:10.1016/j.xpro.2021.101052

Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

STAR Protoc. 2021 Dec 16;3(1):101052. doi: 10.1016/j.xpro.2021.101052. eCollection 2022 Mar 18.

Authors

Keiko Sakamoto^{1

2}, Shubham Goel¹, Atsuko Funakoshi², Tetsuya Honda², Keisuke Nagao¹

Affiliations

¹ Cutaneous Leukocyte Biology Section, Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
² Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.

Abstract

Skin is our body's outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).

Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Flow Cytometry / methods
Keratinocytes*
Mice
Skin*
Suspensions

Substances

Suspensions