This paper describes an easy method to enrich the harvest of adherent mammalian cells at each stage of mitosis (from prometaphase to cytokinesis) by combining Eg5 inhibition using dimethylenastron (DMA) with mitotic shake-off, followed by timed release from the drug.
Keywords: Dimethylenastron (DMA); Eg5; Mammalian; Mitotic synchrony; Survivin.
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