Evaluation of Revogene® Carba C assay for the rapid detection of carbapenemase genes in gram-negative pathogens

Eleonora Riccobono; Chiara Cervini; Fabio Morecchiato; Alberto Antonelli; Gian Maria Rossolini

doi:10.1016/j.diagmicrobio.2021.115614

Evaluation of Revogene® Carba C assay for the rapid detection of carbapenemase genes in gram-negative pathogens

Diagn Microbiol Infect Dis. 2022 Mar;102(3):115614. doi: 10.1016/j.diagmicrobio.2021.115614. Epub 2021 Dec 2.

Authors

Eleonora Riccobono¹, Chiara Cervini², Fabio Morecchiato³, Alberto Antonelli⁴, Gian Maria Rossolini⁵

Affiliations

¹ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Department of Medical Biotechnologies, University of Siena, Siena, Italy.
² Clinical Microbiology and Virology Unit, Careggi University Hospital, Florence, Italy.
³ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
⁴ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Clinical Microbiology and Virology Unit, Careggi University Hospital, Florence, Italy.
⁵ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Clinical Microbiology and Virology Unit, Careggi University Hospital, Florence, Italy. Electronic address: gianmaria.rossolini@unifi.it.

PMID: 34953369
DOI: 10.1016/j.diagmicrobio.2021.115614

Abstract

The rapid detection of carbapenemase-producing microorganisms is crucial for clinical and infection control purposes. Here we evaluated Revogene®Carba C assay on 154 carbapenemase-producing and -nonproducing Gram-negatives. Up to 8 samples per 70 minutes were processed by Revogene®Carba C assay which showed excellent performances with 100% sensitivity, 99.5% specificity,100% NPV, and 97.8% positive predictive value.

MeSH terms

Bacterial Proteins* / genetics
Humans
Sensitivity and Specificity
beta-Lactamases* / genetics

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase