Electrochemical measuring of reactive oxygen species levels in the blood to detect ratio of high-density neutrophils, suitable to alarm presence of cancer in suspicious cases

Zohreh Sadat Miripour; Mina Aminifar; Mohammad Esmaeil Akbari; Fereshteh Abbasvandi; Seyyed Hossein Miraghaie; Parisa Hoseinpour; Mohammad Reza Javadi; Najmeh Dabbagh; Leily Mohajerzadeh; Maryam Kazemi Aghdam; Shahin Shamsian; Hassan Sanati; Mohammad Abdolahad

doi:10.1016/j.jpba.2021.114488

Electrochemical measuring of reactive oxygen species levels in the blood to detect ratio of high-density neutrophils, suitable to alarm presence of cancer in suspicious cases

J Pharm Biomed Anal. 2022 Feb 5:209:114488. doi: 10.1016/j.jpba.2021.114488. Epub 2021 Nov 29.

Affiliations

¹ Nano Bio Electronic Devices Lab, Cancer Research Group, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
² Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ Nano Bio Electronic Devices Lab, Cancer Research Group, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran; ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, P. O. Box 16 15179/64311, Tehran, Iran.
⁴ Nano Bio Electronic Devices Lab, Cancer Research Group, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran; SEPAS Pathology Laboratory, P.O. Box: 1991945391, Tehran, Iran.
⁵ Pathology Surgery Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, P.O. Box. 19395-4719, Tehran, Irang.
⁶ Pediatric Pathology Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, P.O. Box. 19395-4719, Tehran, Iran.
⁷ Pediatric Congenital Hematologic Disorders Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, P.O. Box. 19395-4719, Tehran, Iran.
⁸ ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, P. O. Box 16 15179/64311, Tehran, Iran.
⁹ Nano Bio Electronic Devices Lab, Cancer Research Group, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Institute of Cancer, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: m.abdolahad@ut.ac.ir.

PMID: 34896978
DOI: 10.1016/j.jpba.2021.114488

Abstract

Here for the first time, a real-time electrochemical assay on unprocessed blood was designed to detect the presence of cancer in patients. The system has been based on the recently approved pathway, which indicates that the abundance of immature and mature low-density neutrophils (LDNs) with reduced ROS production in peripheral blood is increased with the presence of active cancer tumors. Reduced ROS/H₂O₂ released from LDNs play the main role in determining the ROS/H₂O₂ levels of peripheral blood. In contrast, HDNs with increased levels of released ROS/H₂O₂ have higher concentrations than LDNs in normal cases. Hence, the reduced level of ROS species in peripheral blood recorded by our carbon nanostructure decorated sensor in less than 30 seconds showed a great pre-warning about the presence of non-treated cancer in patients with suspicious mass who have been sent for further evaluations.

Keywords: Electrochemical assay; Immature and mature neutrophils; Low-density neutrophils; Non-treated cancer; Reactive oxygen species.

MeSH terms

Humans
Hydrogen Peroxide
Neoplasms* / diagnosis
Neutrophils*
Reactive Oxygen Species

Substances

Reactive Oxygen Species
Hydrogen Peroxide