Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor

Proc Natl Acad Sci U S A. 1986 May;83(10):3101-5. doi: 10.1073/pnas.83.10.3101.

Abstract

Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Colony-Stimulating Factors / genetics*
  • Colony-Stimulating Factors / pharmacology
  • Gene Expression Regulation / drug effects
  • Granulocytes
  • Hematopoiesis* / drug effects
  • Humans
  • Macrophages
  • Promoter Regions, Genetic
  • Structure-Activity Relationship

Substances

  • Colony-Stimulating Factors

Associated data

  • GENBANK/M13207