Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Min Liu; Ning Ge; Yongfeng Han; Jamie Reilly; Meimei Yang; Fan Yang; Janusz Krawczyk; Veronica McInerney; Timothy O'Brien; Terence Prendiville; Sanbing Shen

doi:10.1016/j.scr.2021.102607

Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Stem Cell Res. 2021 Dec:57:102607. doi: 10.1016/j.scr.2021.102607. Epub 2021 Nov 24.

Authors

Min Liu¹, Ning Ge², Yongfeng Han¹, Jamie Reilly², Meimei Yang³, Fan Yang⁴, Janusz Krawczyk⁵, Veronica McInerney⁶, Timothy O'Brien⁷, Terence Prendiville⁸, Sanbing Shen⁹

Affiliations

¹ Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, China.
² Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland.
³ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland.
⁴ Hebei Province Children's Hospital, Shijiazhuang, Hebei, China.
⁵ Department of Haematology, Galway University Hospital, Ireland.
⁶ HRB Clinical Research Facility, National University of Ireland (NUI) Galway, Ireland.
⁷ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland. Electronic address: Timothy.OBrien@nuigalway.ie.
⁸ National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin 12, Ireland. Electronic address: Terence.Prendiville@olchc.ie.
⁹ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland. Electronic address: Sanbing.Shen@nuigalway.ie.

PMID: 34844101
DOI: 10.1016/j.scr.2021.102607

Abstract

Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.