EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE

Abstract

In this research, changes in the expression of B-cell lymphoma 2 (BCL2), miR-15-b and miR-16 in human adenocarcinoma gastric cancer cell line (AGS) following the exposure to magnetic flux densities (MFDs) of 0.2 and 2 mT continuously and discontinuously (1.5 h on/1.5 h off) for 18 h were investigated. Changes in the cell viability were evaluated by the MTT assay. Real-time PCR was used to evaluate the expression changes of BCL2, miR-15-b and miR-16. The results showed that extremely low frequency electromagnetic field (ELF-EMF) could significantly reduce the viability of AGS cells in the continuous MFD of 2 mT. The BCL2 expression was significantly decreased following the exposure to continuous MFDs of 0.2 and 2 mT and discontinuous MFD of 2 mT. The expressions of miR-15-b and miR-16 were significantly increased in continuous and discontinuous MFD of 2 mT. According to the results, weak and moderate extremely low-frequency electromagnetic fields can change the expressions of BCL2, miR-15-b and miR-16.