Cystoisospora belli infection in an AIDS patient in China: Need for cautious interpretation of mNGS

Fanli Yi; Fengjun Li; Dongke Chen; Ying Ma

doi:10.1016/j.ijmmb.2021.10.005

Cystoisospora belli infection in an AIDS patient in China: Need for cautious interpretation of mNGS

Indian J Med Microbiol. 2022 Jan-Mar;40(1):163-165. doi: 10.1016/j.ijmmb.2021.10.005. Epub 2021 Nov 10.

Authors

Fanli Yi¹, Fengjun Li², Dongke Chen³, Ying Ma⁴

Affiliations

¹ Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: 897115419@qq.com.
² Department of Laboratory Medicine, The First Hospital of Xinji, Xinji, Hubei, China.
³ Department of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Beijing, China. Electronic address: c-d-k@263.net.
⁴ Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: majiying72@hotmail.com.

PMID: 34772536
DOI: 10.1016/j.ijmmb.2021.10.005

Abstract

Cystoisospora belli (C. belli) is an opportunistic coccidian parasite. This case is the first reported C. belli infection associated with AIDS in China. C. belli infection of this case was diagnosed with the presence of oocysts using direct wet mount and Ziehl-Neelsen acid-fast stain method, and confirmed by polymerase chain reaction (PCR) and Sanger sequencing, ruling out the result of metagenomic next-generation sequencing (mNGS). This case demonstrates that C. belli infection in AIDS could be a potential risk factor for persistent diarrhea, and should not be neglected in non-endemic area and emphaise the necessity of accurate mNGS databases.

Keywords: AIDS; China; Cystoisospora belli.

Publication types

Case Reports

MeSH terms

Acquired Immunodeficiency Syndrome* / complications
High-Throughput Nucleotide Sequencing
Humans
Isosporiasis* / complications
Isosporiasis* / diagnosis
Isosporiasis* / parasitology
Metagenomics
Polymerase Chain Reaction