Re-exploring α-Cyano-4-Hydroxycinnamic Acid as a Reactive Matrix for Selective Detection of Glutathione via MALDI-MS

Liming Guo; Chunsheng Xiao; Jiarui Li; Xi Lin; Nan Zhao; Xinyu Wang; Lejuan Dong; Xinhua Guo

doi:10.1021/jasms.1c00261

Re-exploring α-Cyano-4-Hydroxycinnamic Acid as a Reactive Matrix for Selective Detection of Glutathione via MALDI-MS

J Am Soc Mass Spectrom. 2021 Dec 1;32(12):2837-2841. doi: 10.1021/jasms.1c00261. Epub 2021 Nov 11.

Authors

Liming Guo¹, Chunsheng Xiao², Jiarui Li¹, Xi Lin¹, Nan Zhao¹, Xinyu Wang¹, Lejuan Dong¹, Xinhua Guo^{1

3}

Affiliations

¹ State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
² Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
³ Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China.

PMID: 34761928
DOI: 10.1021/jasms.1c00261

Abstract

Herein, we re-explored α-cyano-4-hydroxycinnamic acid (CHCA) as a reactive matrix for selective and sensitive analysis of glutathione (GSH) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). CHCA efficiently reacted with GSH, and the resulting CHCA-GSH conjugate was readily detected by MALDI-MS without interferences. The detection limit of the CHCA-GSH conjugate decreased to 200 pmol μL^-1, which was 2 orders of magnitude lower than that of pure GSH.Forapplication, CHCA was successfully applied for the detection of GSH, present in HepG2 cell lysates. The results demonstrated detection advantages of simple, high-throughput, and selective and screening of GSH in biological samples by MALDI-MS.

Keywords: cells; glutathione; matrix-assisted laser desorption/ionization mass spectrometry; reactive matrix.