CEBPβ binding directly to the promoter region drives CEBPɑ transcription and improves FABP4 transcriptional activity in adipose tissue of yak (Bos grunniens)

Linsheng Gui; Sayed Haidar Abbas Raza; Boyan Ma; Ahmed A Easa; Fayez Althobaiti; Mustafa Shukry; Mashael Alhumaidi Alotaibi; Tahani Mohamed Ibrahim Al Hazani; Mahmoud A O Dawood; Rajwali Khan; Ali Raza Jahejo; Abdullah F Shater; Ayman H Abd El-Aziz; Guobo Quan; Khawla Hassan Alanbari; Lei Wang

doi:10.1016/j.rvsc.2021.10.018

CEBPβ binding directly to the promoter region drives CEBPɑ transcription and improves FABP4 transcriptional activity in adipose tissue of yak (Bos grunniens)

Res Vet Sci. 2021 Dec:141:174-179. doi: 10.1016/j.rvsc.2021.10.018. Epub 2021 Oct 30.

Authors

Linsheng Gui¹, Sayed Haidar Abbas Raza², Boyan Ma³, Ahmed A Easa⁴, Fayez Althobaiti⁵, Mustafa Shukry⁶, Mashael Alhumaidi Alotaibi⁷, Tahani Mohamed Ibrahim Al Hazani⁸, Mahmoud A O Dawood⁹, Rajwali Khan¹⁰, Ali Raza Jahejo¹¹, Abdullah F Shater¹², Ayman H Abd El-Aziz⁴, Guobo Quan¹³, Khawla Hassan Alanbari¹⁴, Lei Wang¹⁵

Affiliations

¹ State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China; College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China.
² College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China. Electronic address: dr.haiderabbasraza@gmail.com.
³ College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China.
⁴ Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Damanhour, 22511, Egypt.
⁵ Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁶ Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 22511, Egypt.
⁷ Biology Department, College of Science, Jouf University, P.O.Box: 2014, Sakaka, Saudi Arabia.
⁸ Biology Department, College of Science and Humanities, Prince Sattam Bin Abdulaziz University, P.O. Box: 83, Al-Kharj, 11940, Saudi Arabia.
⁹ Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Egypt.
¹⁰ College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
¹¹ Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Sindh 70060 Pakistan.
¹² Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Saudi Arabia.
¹³ Yunnan Animal Science and Veterinary Institute, Kunming 650224, PR China.
¹⁴ Biochemistry Department, College of Science, King Abdoulaziz University, Jeddah, Saudi Arabia.
¹⁵ State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China.

PMID: 34749102
DOI: 10.1016/j.rvsc.2021.10.018

Abstract

Fatty acid binding protein 4 (FABP4) was crucial to fatty acid uptake and intracellular transport. However, the mechanisms regulating yak (Bos grunniens) FABP4 transcription were not determined. In the current study, predominant expression levels of yak FABP4 were identified in subcutaneous fat and longissimus dorsi muscles by quantitative real-time polymerase chain reactions (qPCR). The CCAAT/enhancer binding protein alpha (CEBPα) and myocyte enhancer factor 2A (MEF2A), as transcriptional activator or repressor in the promoter region of FABP4, were confirmed by both site-directed mutagenesis experiment and chromatin immunoprecipitation assay. Additionally, molecular mechanisms of CEBPɑ regulation were analyzed to explore the transcriptional regulatory property of FABP4, which indicated that transcriptional activity of CEBPɑ depended on CCAAT/ enhancer binding protein beta (CEBPβ) transcription factor. Our results demonstrated that CEBPβ binding directly to the promoter region drove CEBPɑ transcription, improving yak FABP4 transcriptional activity in adipocytes. This mechanism expanded the information on the transcriptional regulatory network of adipogenesis.

Keywords: Expression; FABP4; Transcriptional activity; Yak adipocyte.

MeSH terms

Adipogenesis*
Adipose Tissue* / metabolism
Animals
CCAAT-Enhancer-Binding Protein-alpha*
CCAAT-Enhancer-Binding Protein-beta*
Cattle
Fatty Acid-Binding Proteins* / genetics
Fatty Acid-Binding Proteins* / metabolism
Promoter Regions, Genetic

Substances

CCAAT-Enhancer-Binding Protein-alpha
CCAAT-Enhancer-Binding Protein-beta
Fatty Acid-Binding Proteins