New Cu(II) coordination polymer inhibits cervical cancer development by regulating BRCA1 protein via miR-9-5p

J Inorg Biochem. 2022 Jan:226:111655. doi: 10.1016/j.jinorgbio.2021.111655. Epub 2021 Oct 28.

Abstract

A novel Cu(II)-based coordination polymer [chemical composition, {[CuL(CH3CO2)](H2O)(DMF)}n (1, DMF = N,N-dimethylformamide) was successfully prepared via Cu(NO3)2·3H2O reaction with HL ligand in DMF and H2O mixture by using a hetero-donor ligand 4-(bis(4-(4H-1,2,4-triazol-4-yl)phenyl)amino)benzoic acid (HL) featuring carboxylic acid and triazole groups. Reverse transcription-polymerase chain reaction (RT-PCR) was adopted to determine miR-9-5p expression in cervical cancer cells after compound treatment. Bioinformatic analysis and luciferase reporter assay were utilized to confirm miR-9-5p and BRCA1 interaction to discover the potential goal of miR-9-5p in cervical cancer cells. Cell Counting Kit-8 (CCK-8) and reactive oxygen species (ROS) detection kits were adopted to examine cancer cell proliferation and ROS accumulation after compound treatment.

Keywords: BRCA1; Cervical cancer; Cu complex; Proliferation; ROS; miR-9-5p.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • BRCA1 Protein / genetics
  • BRCA1 Protein / metabolism*
  • Cell Proliferation / drug effects*
  • Coordination Complexes / pharmacology*
  • Copper / pharmacology*
  • Female
  • HeLa Cells
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism*
  • Reactive Oxygen Species / metabolism
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / metabolism*

Substances

  • BRCA1 Protein
  • BRCA1 protein, human
  • Coordination Complexes
  • MIRN92 microRNA, human
  • MicroRNAs
  • RNA, Neoplasm
  • Reactive Oxygen Species
  • Copper