Comparison of sulfomethylated lignin from poplar and masson pine on cellulase adsorption and the enzymatic hydrolysis of wheat straw

Mohan Li; Yufeng Yuan; Yangsu Zhu; Bo Jiang; Wenjuan Wu; Shufang Wu; Yongcan Jin

doi:10.1016/j.biortech.2021.126142

Comparison of sulfomethylated lignin from poplar and masson pine on cellulase adsorption and the enzymatic hydrolysis of wheat straw

Bioresour Technol. 2022 Jan:343:126142. doi: 10.1016/j.biortech.2021.126142. Epub 2021 Oct 13.

Authors

Mohan Li¹, Yufeng Yuan¹, Yangsu Zhu², Bo Jiang³, Wenjuan Wu¹, Shufang Wu¹, Yongcan Jin⁴

Affiliations

¹ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China.
² Centre Testing International Group Co., Ltd., Suzhou 215134, China.
³ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China.
⁴ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China. Electronic address: jinyongcan@njfu.edu.cn.

PMID: 34655779
DOI: 10.1016/j.biortech.2021.126142

Abstract

In this work, effects of sulfomethylated lignins (SLs) prepared from masson pine (SL_M) and poplar (SL_P) on enzymatic hydrolysis and cellulase-lignin interaction were comparatively investigated. The results showed that both SL_M and SL_P significantly promoted the substrate enzymatic digestibility. The total sugar yield increased from 38.6% to 74.4% and ∼ 100%, respectively at 10 FPU/g-cellulose of cellulase dosage. The protein content in hydrolysate linearly increased with the addition of SL (0 - 1.6 g/g-substrate lignin), which suggested the competitive adsorption of cellulase may occur to substrate lignin and SLs. Further structural analysis of lignin revealed the high S/(V + H) ratio was directly related to the high enzymatic saccharification efficiency. The strong interaction between SL and cellulase decreased the nonproductive adsorption of cellulase onto substrate lignin and increased the accessibility of cellulase to carbohydrate, which was considered to be the key factor for the improvement of substrate enzymatic digestibility.

Keywords: Cellulase-lignin interaction; Enzymatic hydrolysis; Lignin structure; Protein; Sulfomethylated lignin.

MeSH terms

Adsorption
Cellulase*
Hydrolysis
Lignin*
Triticum

Substances

Lignin
Cellulase