Protective Effects of Catalpol on Limb Motor Function and Ultrastructure of Hippocampal Neurons in Rats with Cerebral Ischemia

Mol Biotechnol. 2022 Feb;64(2):213-219. doi: 10.1007/s12033-021-00407-7. Epub 2021 Sep 29.

Abstract

This study aimed to examine the protective effects of catalpa on ultrastructure of hippocampal neuron and limb motor function in rats with cerebral ischemia. 90 healthy Sprague-Dawley male rats were randomly divided into control (n = 30) and model (n = 60) groups. Cerebral ischemia and hippocampal neurons were induced by occluding the internal carotid artery and injection of high blood glucose, respectively. Model rats were randomly divided into routine (n = 30) and observational (n = 30) groups. Animals in the routine group received edaravone injection (7 mg/kg/day) for 14 days, while rats in the observation group were treated with catalpol (30 mg/kg/day) for 14 days. Limb motor function score, fine motion execution capability, number of hippocampal neurons retained, and the ultrastructure of hippocampal nerve cells were considered at 3, 7, and 14 days after treatments. A significant difference was observed in the mean scores of limb motor function, fine motor execution ability, and the number of hippocampal neurons retained between groups (p < 0.001). Repetitive treatments with catalpol significantly improved the mean number of hippocampal neurons retained (p < 0.01), limb motor function (p < 0.001), and fine motor execution ability scores (p < 0.01) at 3, 7, and 14 days compared to edaravone. Catalpol treatments also improved the ultrastructure morphology of neuronal cells. Catalpa can effectively improve limb motor function and protect hippocampal neuron function in rats with cerebral ischemia.

Keywords: Catalpa; Cerebral ischemia; Hippocampal neurons; Limb motor function; Rats.

MeSH terms

  • Animals
  • Brain Ischemia / drug therapy*
  • Brain Ischemia / pathology
  • Extremities / physiology
  • Hippocampus / drug effects*
  • Hippocampus / pathology
  • Iridoid Glucosides / pharmacology*
  • Male
  • Neurons / drug effects*
  • Neurons / ultrastructure
  • Neuroprotective Agents / pharmacology*
  • Physical Conditioning, Animal
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Iridoid Glucosides
  • Neuroprotective Agents
  • catalpol