Evaluation of Rabbits Liver Fibrosis Using Gd-DTPA-BMA of Dynamic Contrast-Enhanced Magnetic Resonance Imaging

Abstract

Objective: To evaluate the different pharmacokinetic parameters of the DCE-MRI method on diagnosing and staging of rabbits' liver fibrosis.

Methods: We had performed DCE-MRI for rabbits that had been divided into the experiment group and the control group. Then, rabbits' images were transferred to a work station to get three parameters such as K _trans, K _ep, and V _e, which had been measured to calculate. After data were analyzed, ROC analyses were performed to assess the diagnostic performance of K _trans, K _ep, and V _e to judge liver fibrosis.

Results: The distribution of the different liver fibrosis group was as follows: F1, n = 8; F2, n = 9; F3, n = 6; F4, n = 5. No fibrosis was deemed as F0, n = 6. K _ep is statistically significant (P < 0.05) for F0 and mild liver fibrosis stage, and the K _ep shows AUC of 0.814. Three parameters are statistically significant for F0 and advanced liver fibrosis stage (K _trans and K _ep, P < 0.01; V _e, P < 0.05), and the K _trans shows AUC of 0.924; the K _ep shows AUC of 0.909; the V _e shows AUC of 0.848; K _trans and K _ep are statistically significant for mild and advanced liver fibrosis stages (K _trans, P < 0.01; K _ep, P < 0.05), and the K _trans shows AUC of 0.840; the K _ep shows AUC of 0.765. Both K _trans and K _ep are negatively correlated with the liver fibrosis stage. V _e is positively correlated with the liver fibrosis stage.

Conclusion: K _trans is shown to be the best DCE parameter to distinguish the fibrotic liver from the normal liver and mild and advanced fibrosis. On the contrary, K _ep is moderate and V _e is worst. And K _ep is a good DCE parameter to differentiate mild fibrosis from the normal liver.