Metabolism perturbation Causedby the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate ofEubacterium limosumKIST612

Bioresour Technol. 2021 Dec:341:125879. doi: 10.1016/j.biortech.2021.125879. Epub 2021 Sep 4.

Abstract

Microbial conversion of carbon monoxide (CO) to acetate is a promising upcycling strategy for carbon sequestration. Herein, we demonstrate that CO conversion and acetate production rates of Eubacterium limosum KIST612 strain can be improved by in silico prediction and in vivo assessment. The mimicked CO metabolic model of KIST612 predicted that overexpressing the CO dehydrogenase (CODH) increases CO conversion and acetate production rates. To validate the prediction, we constructed mutant strains overexpressing CODH gene cluster and measured their CO conversion and acetate production rates. A mutant strain (ELM031) co-overexpressing CODH, coenzyme CooC2 and ACS showed a 3.1 × increased specific CO oxidation rate as well as 1.4 × increased specific acetate production rate, compared to the wild type strain. The transcriptional and translational data with redox balance analysis showed that ELM031 has enhanced reducing potential from up-regulation of ferredoxin and related metabolism directly linked to energy conservation.

Keywords: Acetogen; CO dehydrogenase; Eubacterium limosum; Homologous overexpression; Metabolic change.

MeSH terms

  • Acetates
  • Acetyl Coenzyme A
  • Aldehyde Oxidoreductases* / genetics
  • Carbon Monoxide*
  • Eubacterium
  • Multienzyme Complexes

Substances

  • Acetates
  • Multienzyme Complexes
  • Acetyl Coenzyme A
  • Carbon Monoxide
  • Aldehyde Oxidoreductases
  • carbon monoxide dehydrogenase

Supplementary concepts

  • Eubacterium limosum