DrFLINC Contextualizes Super-resolution Activity Imaging

J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13.

Abstract

Super-resolution activity imaging maps the biochemical architecture of living cells yet currently overlooks the locations of collaborating regulators/effectors. Building on the fluorescence fluctuation increase by contact (FLINC) principle, here we devise Dronpa-chromophore-removed FLINC (DrFLINC), where the nonfluorescent Dronpa can nevertheless enhance TagRFP-T fluorescence fluctuations. Exploiting DrFLINC, we develop a superior red label and a next-generation activity sensor for context-rich super-resolution biosensing.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Green Fluorescent Proteins / chemistry*
  • HeLa Cells
  • Humans

Substances

  • Fluorescent Dyes
  • Green Fluorescent Proteins