C3 and C4 complement levels in AQP4-IgG-positive NMOSD and in MOGAD

Florence Pache; Marius Ringelstein; Orhan Aktas; Ingo Kleiter; Sven Jarius; Nadja Siebert; Judith Bellmann-Strobl; Friedemann Paul; Klemens Ruprecht

doi:10.1016/j.jneuroim.2021.577699

C3 and C4 complement levels in AQP4-IgG-positive NMOSD and in MOGAD

J Neuroimmunol. 2021 Nov 15:360:577699. doi: 10.1016/j.jneuroim.2021.577699. Epub 2021 Aug 24.

Authors

Florence Pache¹, Marius Ringelstein², Orhan Aktas³, Ingo Kleiter⁴, Sven Jarius⁵, Nadja Siebert⁶, Judith Bellmann-Strobl⁶, Friedemann Paul⁷, Klemens Ruprecht⁸

Affiliations

¹ Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Neurology, Berlin, Germany.
² Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany; Department of Neurology, Center for Neurology and Neuropsychiatry, LVR-Klinikum Düsseldorf, Düsseldorf, Germany.
³ Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.
⁴ St. Josef-Hospital, Department of Neurology, Ruhr-University Bochum, Bochum, Germany; Marianne-Strauß-Klinik, Behandlungszentrum Kempfenhausen für Multiple Sklerose Kranke gGmbH, Berg, Germany.
⁵ Molecular Neuroimmunology Group, Department of Neurology, University of Heidelberg, Heidelberg, Germany.
⁶ NeuroCure Clinical Research Center, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany; Experimental and Clinical Research Center, Charité - Universitätsmedizin Berlin, Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany; Humboldt-Universität zu Berlin, Berlin Institute of Health, Berlin, Germany.
⁷ Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Neurology, Berlin, Germany; NeuroCure Clinical Research Center, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany; Experimental and Clinical Research Center, Charité - Universitätsmedizin Berlin, Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
⁸ Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Neurology, Berlin, Germany. Electronic address: klemens.ruprecht@charite.de.

PMID: 34464830
DOI: 10.1016/j.jneuroim.2021.577699

Abstract

While complement-dependent cytotoxicity (CDC) is a known effector mechanism in aquaporin-4-immunoglobulin (Ig)G-positive (AQP4-IgG+) neuromyelitis optica spectrum disorder (NMOSD), the role of CDC in myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is less clear. We determined complement C3 and C4 plasma concentrations in patients with clinically stable AQP4-IgG+ NMOSD (n = 16), MOGAD (n = 15), early multiple sclerosis (MS, n = 19) and in healthy controls (HC, n = 18). C4 was lower in AQP4-IgG+ NMOSD than in MOGAD, MS and HC (p < 0.05, pairwise comparisons). C3 was lower in AQP4-IgG+ NMOSD than in MS (p = 0.034). These findings suggest subtle complement consumption in clinically stable AQP4-IgG+ NMOSD, but not in MOGAD.

Keywords: Antibodies; Aquaporin-4; C3; C4; Complement; Myelin oligodendrocyte glycoprotein; Neuromyelitis optica spectrum disorder.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aquaporin 4 / immunology*
Autoantibodies / immunology*
Complement Activation
Complement C3 / analysis*
Complement C4 / analysis*
Cytotoxicity, Immunologic
Demyelinating Autoimmune Diseases, CNS / immunology*
Female
Humans
Immunoglobulin G / immunology*
Male
Middle Aged
Multiple Sclerosis, Relapsing-Remitting / immunology*
Myelin-Oligodendrocyte Glycoprotein / immunology*
Neuromyelitis Optica / blood
Neuromyelitis Optica / immunology*
Prospective Studies

Substances

AQP4 protein, human
Aquaporin 4
Autoantibodies
C3 protein, human
Complement C3
Complement C4
Immunoglobulin G
MOG protein, human
Myelin-Oligodendrocyte Glycoprotein