Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae

Yuyu Huang; Haoran Yu; Min Liang; Shule Hou; Junmin Chen; Fan Zhang; Xiayu Sun; Huan Jia; Jun Yang

doi:10.2147/DDDT.S318117

Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae

Drug Des Devel Ther. 2021 Aug 11:15:3443-3450. doi: 10.2147/DDDT.S318117. eCollection 2021.

Authors

Yuyu Huang^#^{1

2}, Haoran Yu^#³, Min Liang^{1

2}, Shule Hou^{1

2}, Junmin Chen^{1

2}, Fan Zhang^{1

2}, Xiayu Sun^{1

2}, Huan Jia^{2

4

5}, Jun Yang^{1

2

5}

Affiliations

¹ Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, People's Republic of China.
² Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases (14DZ2260300), Shanghai, 200125, People's Republic of China.
³ ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, People's Republic of China.
⁴ Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, People's Republic of China.
⁵ Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, 200031, People's Republic of China.

^# Contributed equally.

Abstract

Objective: To investigate the hearing protection outcomes of different drug-eluting analog electrode arrays implanted into guinea pig cochleae.

Methods: Sixty guinea pigs were randomly divided into a negative control group and five experimental groups implanted separately with blank (drug carrier), dexamethasone (DXM), aracytine (Ara-C), Ara-C+DXM, and nicotinamide adenine dinucleotide (NAD+) eluting analog electrode arrays. Micro CT was used to supervise the surgical procedure. Auditory brainstem response (ABR) thresholds of the guinea pigs were measured and analyzed.

Results and conclusions: Compared with the negative control, all other groups showed a significant increase in ABR threshold (p<0.001) after surgery. Among them, there was no obvious difference between the blank (0 vs 90 days: 59.70±10.57 vs 64.60±9.47 dB SPL) and the NAD+ group (0 vs 90 days: 59.90±9.87 vs 64.70±8.65 dB SPL). On the other hand, the ABR thresholds in the DXM (0 days: 58.10±10.73 dB SPL; 90 days: 51.70±9.07 dB SPL) and the Ara-C group (0 days: 59.00±10.05 dB SPL; 90 days: 51.60±8.48 dB SPL) decreased significantly compared with the former two groups (p<0.001). However, the Ara-C+DXM group showed no further benefit (p>0.05). In addition, a significantly higher survival rate of spiral ganglion neurons in cochleae was observed in the Ara-C and/or DXM groups.

Keywords: cochlear implant; drug-eluting electrode; guinea pig; hearing protection.

Publication types

Comparative Study

MeSH terms

Animals
Cochlear Implants*
Cytarabine / administration & dosage
Dexamethasone / administration & dosage
Electrodes, Implanted
Evoked Potentials, Auditory, Brain Stem / drug effects*
Female
Guinea Pigs
Hearing Loss / prevention & control*
NAD / administration & dosage
Polymers / chemistry

Substances

Polymers
Cytarabine
NAD
Dexamethasone

Grants and funding

This work was supported by the Shanghai Natural Science Foundation (17ZR1416100), Shanghai Academic Leadership Program (16XD1402200), and National Natural Science Foundation of China (81200742, 81800903).