Quantitative image analysis method for detection of nitrite with cyanine dye-NaYF4:Yb,Tm@NaYF4 upconversion nanoparticles composite luminescent probe

Hongqi Chen; Wei Tang; Yunchun Liu; Lun Wang

doi:10.1016/j.foodchem.2021.130660

Quantitative image analysis method for detection of nitrite with cyanine dye-NaYF₄:Yb,Tm@NaYF₄ upconversion nanoparticles composite luminescent probe

Food Chem. 2022 Jan 15:367:130660. doi: 10.1016/j.foodchem.2021.130660. Epub 2021 Aug 3.

Authors

Hongqi Chen¹, Wei Tang², Yunchun Liu², Lun Wang³

Affiliations

¹ Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: hq80chen@mail.ahnu.edu.cn.
² Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
³ Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: wanglun@mail.ahnu.edu.cn.

PMID: 34390907
DOI: 10.1016/j.foodchem.2021.130660

Abstract

In this work, a quantitative image analysis method based on cyanine dye-upconversion nanoparticles composite luminescent nanoprobe for the detection of nitrite was developed. The nanoprobe was constructed by combining the NaYF₄:Yb,Tm@NaYF₄ upconversion nanoparticles (UCNPs) and the new cyanine dye IR-790. The upconversion nanoparticles transferred energy to IR-790, resulting in the luminescence quenching, while the luminescence of UCNPs was recovered after adding NO₂^-. The increase in photons was related to the concentration of NO₂^-. Under the optimal experimental conditions, the detection range was 0.20-140 μM and the limit of detection was 0.030 μM. The measurement for NO₂^- can be completed in 29 min. The method has the characteristics of fast response (~0.1 s), low sample consumption (10 μL) and powerful data support (550 frame time series images). Furthermore, the quantitative image analysis method was successfully applied for the analysis of nitrite in environmental water and food samples.

Keywords: Cyanine dye; NaYF(4):Yb; Nitrite; Quantitative image analysis; Tm@NaYF(4) upconversion nanoparticles.

MeSH terms

Coloring Agents
Luminescence*
Nanoparticles*
Nitrites

Substances

Coloring Agents
Nitrites