Multiplexed Raman Imaging in Tissues and Living Organisms

Travis M Shaffer; Sanjiv S Gambhir

doi:10.1007/978-1-0716-1593-5_21

Multiplexed Raman Imaging in Tissues and Living Organisms

Methods Mol Biol. 2021:2350:331-340. doi: 10.1007/978-1-0716-1593-5_21.

Authors

Travis M Shaffer¹, Sanjiv S Gambhir^{2

3

4}

Affiliations

¹ Department of Radiology, Stanford University, Stanford, CA, USA. trvsshaffer@gmail.com.
² Department of Radiology, Stanford University, Stanford, CA, USA.
³ Department of Bioengineering, Stanford University, Stanford, CA, USA.
⁴ Bio-X Program, Molecular Imaging Program at Stanford (MIPS), Canary Center for Early Cancer Detection, Stanford, CA, USA.

PMID: 34331295
DOI: 10.1007/978-1-0716-1593-5_21

Abstract

Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are ideal multiplexing probes for in vivo imaging and tissue staining. Their remarkable sensitivity and unique Raman molecular fingerprint results in minimal background compared to other optical modalities. These characteristics also allow multiplexing down to the attomolar concentration. Here we describe the synthesis and in vivo multiplexing application of a SERS NP library.

Keywords: Multiplexing; Nanoparticles; Raman; Resonance; SERS.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Data Analysis
Ligands
Metal Nanoparticles
Molecular Imaging / methods*
Molecular Probes
Spectrum Analysis, Raman / methods*
Workflow

Substances

Ligands
Molecular Probes

Abstract

Publication types

MeSH terms

Substances

Grants and funding