The Power of Systems Biology: Insights on Lupus Nephritis from the Accelerating Medicines Partnership

Andrea Fava; Soumya Raychaudhuri; Deepak A Rao

doi:10.1016/j.rdc.2021.04.003

The Power of Systems Biology: Insights on Lupus Nephritis from the Accelerating Medicines Partnership

Rheum Dis Clin North Am. 2021 Aug;47(3):335-350. doi: 10.1016/j.rdc.2021.04.003. Epub 2021 Jun 16.

Authors

Andrea Fava¹, Soumya Raychaudhuri², Deepak A Rao³

Affiliations

¹ Division of Rheumatology, Johns Hopkins University, 1830 East Monument Street, Suite 7500, Baltimore, MD 21205, USA. Electronic address: afava1@jh.edu.
² Division of Rheumatology, Inflammation, Immunity, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Center for Data Sciences, Brigham and Women's Hospital, Building for Transformative Medicine, 60 Fenwood Road, Boston, MA 02115, USA; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, USA; Centre for Genetics and Genomics Versus Arthritis, Centre for Musculoskeletal Research, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester, UK. Electronic address: https://twitter.com/soumya_boston.
³ Division of Rheumatology, Inflammation, Immunity, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: darao@bwh.harvard.edu.

PMID: 34215367
DOI: 10.1016/j.rdc.2021.04.003

Abstract

The Accelerating Medicines Partnership (AMP) SLE Network united resources from academic centers, government, nonprofit, and industry to accelerate discovery in lupus nephritis (LN). The AMP SLE Network developed a set of protocols for high-throughput analyses to systematically study kidney tissue, urine, and blood in LN. This article summarizes approaches and results from phase 1 of AMP SLE Network effort, including single cell RNA-seq analysis of LN kidney biopsies, cellular and proteomic studies of LN urine, and mass cytometry immunophenotyping of blood cells. This work provides a framework to guide studies of the clinical implications of active cellular/molecular pathways in LN.

Keywords: AMP; Accelerating medicines partnership; Biomarker; Lupus nephritis; Proteomics; SLE; Transcriptomics; scRNA-seq.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Biomarkers
Biopsy
Humans
Lupus Nephritis* / drug therapy
Proteomics
Systems Biology

Substances

Biomarkers

Abstract

Publication types

MeSH terms

Substances

Grants and funding