Discovery of eudesmane-type sesquiterpenoids with neuroprotective effects from the roots of Chloranthus serratus

Abstract

Seven eudesmane-type sesquiterpenoids, including three pairs of racemic compounds (1a-3a and 1b-3b) and a sesquiterpenoid lactone (4), were obtained from the roots of Chloranthus serratus. The structures of these sesquiterpenoids were characterized based on spectroscopic analyses, ECD calculations, and X-ray diffraction experiment. Neuroprotection assays of the isolated eudesmane-type sesquiterpenoids were conducted on H₂O₂ damaged PC12 cells. At the concentration of 10 μM, compounds 1b and 4 increased cell viability from 54.8 ± 3.3% to 76.8 ± 2.3 and 72.7 ± 8.2%, respectively.

Keywords: Chloranthus serratus; Epimer; Eudesmane-type sesquiterpenoids; Neuroprotective activities; Racemic compounds.