IgG Antibody Responses Are Preferential Compared With IgM for Use as Serological Markers for Detecting Recent Exposure to Plasmodium vivax Infection

Rhea J Longley; Michael T White; Jessica Brewster; Zoe S J Liu; Caitlin Bourke; Eizo Takashima; Matthias Harbers; Wai-Hong Tham; Julie Healer; Chetan E Chitnis; Wuelton Monteiro; Marcus Lacerda; Jetsumon Sattabongkot; Takafumi Tsuboi; Ivo Mueller

doi:10.1093/ofid/ofab228

IgG Antibody Responses Are Preferential Compared With IgM for Use as Serological Markers for Detecting Recent Exposure to Plasmodium vivax Infection

Open Forum Infect Dis. 2021 May 22;8(6):ofab228. doi: 10.1093/ofid/ofab228. eCollection 2021 Jun.

Authors

Rhea J Longley^{1

2}, Michael T White³, Jessica Brewster¹, Zoe S J Liu^{1

2}, Caitlin Bourke^{1

2}, Eizo Takashima⁴, Matthias Harbers⁵, Wai-Hong Tham^{2

6}, Julie Healer^{2

6}, Chetan E Chitnis⁷, Wuelton Monteiro^{8

9}, Marcus Lacerda¹⁰, Jetsumon Sattabongkot¹¹, Takafumi Tsuboi⁴, Ivo Mueller^{1

2

3}

Affiliations

¹ Population Health and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
² Department of Medical Biology, University of Melbourne, Parkville, Australia.
³ Malaria Parasites and Hosts Unit, Department of Parasites and Insect Vectors, Institut Pasteur, Paris, France.
⁴ Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan.
⁵ CellFree Sciences Co., Ltd, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
⁶ Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
⁷ Malaria Parasite Biology and Vaccines, Department of Parasites and Insect Vectors, Institut Pasteur, Paris, France.
⁸ Fundacão de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, Brazil.
⁹ Universidade do Estado do Amazonas, Manaus, Brazil.
¹⁰ Instituto Leônicas & Maria Deane (Fiocruz), Manaus, Brazil.
¹¹ Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Abstract

To achieve malaria elimination, new tools are required to explicitly target Plasmodium vivax. Recently, a novel panel of P. vivax proteins were identified and validated as serological markers for detecting recent exposure to P. vivax within the last 9 months. In order to improve the sensitivity and specificity of these markers, immunoglobulin M (IgM) in addition to immunoglobulin G (IgG) antibody responses were compared with a down-selected panel of 20 P. vivax proteins. IgM was tested using archival plasma samples from observational cohort studies conducted in malaria-endemic regions of Thailand and Brazil. IgM responses to these proteins generally had poorer classification performance than IgG.

Keywords: IgG; IgM; Plasmodium vivax; antibody; malaria; serological exposure markers; serology; surveillance.

Abstract

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