Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts

STAR Protoc. 2021 May 26;2(2):100553. doi: 10.1016/j.xpro.2021.100553. eCollection 2021 Jun 18.

Abstract

Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol to establish CAFs from surgically resected tissues. CAFs could be a vital tool for understanding the microenvironment and its impact on tumor progression and metastasis. Moreover, we generated inflammation-induced senescent fibroblasts that more closely mimic the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cancer-Associated Fibroblasts / cytology*
  • Cancer-Associated Fibroblasts / metabolism
  • Cellular Senescence*
  • Cytokines / metabolism
  • Humans
  • Inflammation Mediators / metabolism
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / surgery*
  • Tumor Microenvironment

Substances

  • Cytokines
  • Inflammation Mediators